Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: On the interface of fatty acid metabolism: A crosstalk between fatty acid ω-hydroxylase CYP4F11 and fatty acid desaturase 2 in non—small cell lung cancer

doi: 10.1016/j.dmd.2025.100221

Figure Lengend Snippet: CYP4F11 was overexpressed (OE) in NCI-H460 cells (top panel) and NCI-H1437 cells (bottom panel). The GFP-CYP4F11 overexpression was confirmed via Western blot analysis indicated by a band at 88 kDa (A, D). A CYP4F11 overexpression promotes the proliferation of both cell lines as indicated by the MTT assay (B, E) and cell counting assay (C, F). All data were normalized and analyzed with a 2-way ANOVA test ( n = 3–4; ** P ≤ .01; ***P ≤ .001). FADS2, fatty acid desaturase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: The NSCLC cell lines NCI-H460 and NCI-H1437 were purchased from ATCC.

Techniques: Over Expression, Western Blot, MTT Assay, Cell Counting

Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: On the interface of fatty acid metabolism: A crosstalk between fatty acid ω-hydroxylase CYP4F11 and fatty acid desaturase 2 in non—small cell lung cancer

doi: 10.1016/j.dmd.2025.100221

Figure Lengend Snippet: A stable CYP4F11 overexpression (OE) promoted the migration of NCI-H460 (top panel) and NCI-1437 (bottom panel) cells as assessed by the wound healing assay (A, C). The wound closing was monitored and quantified between 0 and 24 hours (B, D). All data were normalized and analyzed with a 2-way ANOVA test ( n = 3; ** P ≤ .01; *** P ≤ .001; **** P ≤ .0001).

Article Snippet: The NSCLC cell lines NCI-H460 and NCI-H1437 were purchased from ATCC.

Techniques: Over Expression, Migration, Wound Healing Assay

Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: On the interface of fatty acid metabolism: A crosstalk between fatty acid ω-hydroxylase CYP4F11 and fatty acid desaturase 2 in non—small cell lung cancer

doi: 10.1016/j.dmd.2025.100221

Figure Lengend Snippet: RNA sequencing was conducted to compare the transcription between NCI-H460 siCYP4F11 and siControl cells. Differentially expressed genes were visualized using a volcano plot (A). Top downregulated genes were summarized in a heatmap (B). Our subsequent analysis focused on the fatty acid desaturase 2 (FADS2, red boxed), which catalyzes the first and rate-limiting step in arachidonic acid synthesis from linoleic acid via desaturation at C6 yielding dihomo-γ-linolenic acid. Fatty acid desaturase 1 (FADS1) catalyzes the final desaturation resulting in arachidonic acid (C).

Article Snippet: The NSCLC cell lines NCI-H460 and NCI-H1437 were purchased from ATCC.

Techniques: RNA Sequencing

Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: On the interface of fatty acid metabolism: A crosstalk between fatty acid ω-hydroxylase CYP4F11 and fatty acid desaturase 2 in non—small cell lung cancer

doi: 10.1016/j.dmd.2025.100221

Figure Lengend Snippet: The FADS2 mRNA level decreases after knocking down CYP4F11 via siRNA in both NCI-H460 (top panel) and NCI-1437 cells (bottom panel), whereby the mRNA level of CYP4F11 and FADS2 was normalized to GAPDH, respectively (A, D). The FADS2 protein level decreased after CYP4F11 knockdown in both cell lines, whereby the protein level of CYP4F11 and FADS2 was normalized to GAPDH, respectively (B, C, E, F). All data were normalized and analyzed with a Student’s t test ( n = 3; ** P ≤ .01; *** P ≤ .001; **** P ≤ .0001). FADS2, fatty acid desaturase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The NSCLC cell lines NCI-H460 and NCI-H1437 were purchased from ATCC.

Techniques: Knockdown

Journal: Drug metabolism and disposition: the biological fate of chemicals

Article Title: On the interface of fatty acid metabolism: A crosstalk between fatty acid ω-hydroxylase CYP4F11 and fatty acid desaturase 2 in non—small cell lung cancer

doi: 10.1016/j.dmd.2025.100221

Figure Lengend Snippet: The FADS2 mRNA level increases after overexpression (OE) of CYP4F11 in both NCI-H460 (top panel) and NCI-1437 cells (bottom panel) whereby the mRNA level of CYP4F11 and FADS2 was normalized to GAPDH, respectively (A, D). The FADS2 protein level increased after CYP4F11 overexpression in both cell lines, whereby the protein level of CYP4F11 and FADS2 was normalized to GAPDH, respectively (B, C, E, F). All data were normalized and analyzed with a Student’s t test ( n = 3; ** P ≤ .01; *** P ≤ .001; **** P ≤ .0001). FADS2, fatty acid desaturase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The NSCLC cell lines NCI-H460 and NCI-H1437 were purchased from ATCC.

Techniques: Over Expression